tmb substrate (Surmodics IVD)

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Surmodics IVD tmb substrate

CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.

Tmb Substrate, supplied by Surmodics IVD, used in various techniques. Bioz Stars score: 95/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/one+component+hrp+microwell+substrate/TMB+One+Component+HRP+Microwell+Substrate/pmc13106428-247-4-6
Average 95 stars, based on 176 article reviews

tmb substrate - by Bioz Stars, 2026-09

95/100 stars

Images

1) Product Images from "Immunization with detoxified TNFα elicits neutralizing antibodies and ameliorates inflammatory shock and autoimmune arthritis in mice"

Article Title: Immunization with detoxified TNFα elicits neutralizing antibodies and ameliorates inflammatory shock and autoimmune arthritis in mice

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2026.1801223

Figure Legend Snippet: CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off with PBST. Captured biotinylated mTNFα was detected with streptavidin-HRP and TMB substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.

Techniques Used: Blocking Assay, Binding Assay, Vaccines, Enzyme-linked Immunosorbent Assay, Inhibition, Incubation

Expressing:

Article Title: Residues within the C-Terminal Arm of the Herpes Simplex Virus 1 Glycoprotein B Ectodomain Contribute to Its Refolding during the Fusion Step of Virus Entry
Article Snippet: .. Expression was determined by adding BioFx TMB one-component HRP microwell substrate (SurModics, Eden Prarie, MN) and measuring the absorbance at 370 nm. .. To probe gB conformation by CELISA, CHO-K1 cells seeded overnight in six-well plates (106 cells/well) were transfected with DNA encoding gB or empty vector (2.2 g/well) using 7 l Lipofectamine 2000/well (1 ml/well, total volume).

Article Title: Residues within the C-Terminal Arm of the Herpes Simplex Virus 1 Glycoprotein B Ectodomain Contribute to Its Refolding during the Fusion Step of Virus Entry
Article Snippet: .. Expression was determined by adding BioFx TMB one-component HRP microwell substrate (SurModics, Eden Prarie, MN) and measuring the absorbance at 370 nm. .. To probe gB conformation by CELISA, CHO-K1 cells seeded overnight in six-well plates (10 6 cells/well) were transfected with DNA encoding gB or empty vector (2.2 µg/well) using 7 μl Lipofectamine 2000/well (1 ml/well, total volume).

Control:

Article Title: Identification of direct negative cross-talk between the SLIT2 and bone morphogenetic protein–Gremlin signaling pathways
Article Snippet: .. The wells containing immobilized SLIT2 and control wells were then incubated with indicated amounts of Gremlin (Sigma, SRP3285) overnight, followed by incubation with anti-Gremlin antibody (Cell Signaling, 4383S, lot 1, 1:100), anti-rabbit-IgG horseradish peroxidase-linked whole antibody (GE Healthcare, NA934V, lot 9653124, 1:1000), and 3,3′, 5,5′-tetramethylbenzidine one-component HRP microwell substrate (Surmodics, TMBW-1000-01). ..

Incubation:

Article Title: The neutralizing antibody, LY-CoV555, protects against SARS-CoV-2 infection in nonhuman primates
Article Snippet: After incubation, the plate was washed to remove unbound material, and mouse anti-human IgG Fc-HRP (horseradish peroxidase) (10 ng/ml; SouthernBiotech, catalog no. 9040-05) was added as detection reagent. .. After incubation, unbound enzyme was washed away and BioFX 3,3′,5,5′-tetramethylbenzidine one-component HRP microwell substrate (SurModics, catalog no. TMBW-0100-01 or equivalent) was added to the wells. .. Color development was stopped by the addition of phosphoric acid (Fisher Chemical, catalog no. A260-500 or equivalent), and the optical density was measured at 450 nm with wavelength correction set to 650 nm.

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